Tsunagi Protein Plays a Critical Role in the Development of the Reproductive Organs and Eggs of Schistosoma japonicum
Tsunagi is an evolutionally conserved RNA-binding protein that is required for the maintenance of oogenesis and masculinization of the germ line in many animal models．We speculated that Tsunagi may also regulate reproductive organ development in Schistosoma japonicum．We introduced S．japonicum Tsunagi double-stranded RNA into schistosomula by electroporation．These transfected schistosomula were cultured in vitro，and the mRNA and protein levels of the target gene were found to be significantly suppressed by interference．Further，BALB／c mice were infected witll the transfected SChistosomula for 6 weeks and sacrificed for harvesting adult worins．We found that silencing of S．japonicum Tsunagi led to defects in the development of reproductive organs in both male and female worms．Moreover，it affected the production and development of eggs．Our study indicates that Tsunagi plays a critical role in the development of reproductive organs and eggs in Schistosoma japonicum．
Cui-Ping Ren Peng Zhang Wei-Na Zhang Da-Ke Huang Xue-Mei Jia Li Gui Miao Liu Ji-Jia Shen
Department of Microbiology and Parasitology,Anhui Provincial Laboratory of Microbiology and Parasito Comprehensive Laboratory,School of Basic Medical Science，Anhui Medical University,81＃ Meishan Road,H Department of Microbiology and Parasitology，Anhui Medical University，81＃ Meishan Road，Hefei，Anhui 23
国内会议
2012年安徽省医学会微生物与免疫学、热带病与寄生虫病学会换届改选与学术研讨会
黄山
英文
11-22
2012-08-11（万方平台首次上网日期，不代表论文的发表时间）