Chrominance to Dimension: A Real-Time Method for Measuring the Size of Single Gold Nanoparticles
Noble metal nanoparticles have excellentoptical and chemical properties and are widely used in optics,sensors, and biomedicines. The inherent characteristics ofmetal nanoparticles, particularly their size, play important rolesin their applications. The ability to readily measure the size ofsingle nanomaterials on-site is crucial to the rapid development of single-particle sensors. In this study, we developed a facile andreal-time method for estimating the diameter of single gold nanoparticles (GNPs) that range from 35 to 110 nm in diameter; thistechnique uses the chrominance of the GNP’s plasmon resonance scattering light that is captured by a dark-field microscope(DFM). The RGB (three primary colors, red, green, and blue) chrominance information from the dark-field image can bedirectly converted into the diameters of the GNPs using the relationship between the particle size and the scattering light peakwavelength; this conversion was carried out using Matlab program based on an RGB-To-Wavelength (RTW) process. Thisapproach is more convenient, less time-consuming, and enables observation under arbitrary conditions compared to the scanningelectron microscopy (SEM) technique. The differences between the diameters of the GNPs that were calculated using thismethod and those that were measured using SEM were less than 5 nm. The RTW method has also been applied in themonitoring of the refractive index of the media surrounding the GNPs, and their dynamic acting within cells in real-time.
Chao Jing Zhen Gu Yi-Lun Ying Da-Wei Li Lei Zhang Yi-Tao Long
Key Laboratory for Advanced Materials & Department of Chemistry,East China University of Science and Key Laboratory for Advanced Materials & Department of Chemistry,East China University of Science and
国内会议
2012上海市研究生学术论坛暨第三届上海交通大学医(理)工研究生学术论坛
上海
英文
1-8
2012-08-15（万方平台首次上网日期，不代表论文的发表时间）