Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging
Background: In recent years, near-infrared fluorescence (NIRF)-labeled iron nanoparticles have been synthesizedand applied in a number of applications, including the labeling of human cells for monitoring the engraftmentprocess, imaging tumors, sensoring the in vivo molecular environment surrounding nanoparticles and tracing theirin vivo biodistribution. These studies demonstrate that NIRF-labeled iron nanoparticles provide an efficient probefor cell labeling. Furthermore, the in vivo imaging studies show excellent performance of the NIR fluorophores.However, there is a limited selection of NIRF-labeled iron nanoparticles with an optimal wavelength for imagingaround 800 nm, where tissue autofluorescence is minimal. Therefore, it is necessary to develop additionalalternative NIRF-labeled iron nanoparticles for application in this area.Results: This study manufactured 12-nm DMSA-coated Fe3O4 nanoparticles labeled with a near-infraredfluorophore, IRDye800CW (excitation/emission, 774/789 nm), to investigate their applicability in cell labeling and invivo imaging. The mouse macrophage RAW264.7 was labeled with IRDye800CW-labeled Fe3O4 nanoparticles atconcentrations of 20, 30, 40, 50, 60, 80 and 100 μg/ml for 24 h. The results revealed that the cells were efficientlylabeled by the nanoparticles, without any significant effect on cell viability. The nanoparticles were injected intothe mouse via the tail vein, at dosages of 2 or 5 mg/kg body weight, and the mouse was discontinuously imagedfor 24 h. The results demonstrated that the nanoparticles gradually accumulated in liver and kidney regionsfollowing injection, reaching maximum concentrations at 6 h post-injection, following which they were graduallyremoved from these regions. After tracing the nanoparticles throughout the body it was revealed that they mainlydistributed in three organs, the liver, spleen and kidney. Real-time live-body imaging effectively reported thedynamic process of the biodistribution and clearance of the nanoparticles in vivo.Conclusion: IRDye800CW-labeled Fe3O4 nanoparticles provide an effective probe for cell-labeling and in vivoimaging.
Yong Hou Yingxun Liu Zhongping Chen Ning Gu Jinke Wang
State key Laboratory of Bioelectronics,Southeast University,Nanjing 210096,China Full list of author State key Laboratory of Bioelectronics,Southeast University,Nanjing 210096,China
国内会议
2012上海市研究生学术论坛暨第三届上海交通大学医(理)工研究生学术论坛
上海
英文
1-14
2012-08-15（万方平台首次上网日期，不代表论文的发表时间）