Preparation of Surface Modified Magnetic Nanoparticles and Its Application in Pseudomonas aeruginosa Rapidly Detection

摘要：

The purpose of this paper is to study the preparation of magnetic nanoparticles (MNPs)by solvothermal method with PEG-4000 as surfactant, the surface modification of the MNPs byseveral steps, rapidly detection method of pseudomonas aeruginosa based on magnetic separationand chemiluminescence. The results shown that we prepared a kind of MNPs with uniform shapand good dispersed, and the sizes of MNPs can be controlled by adjusted the dosage ofFeCl3·6H2O. SEM, TEM and FTIR showed that the several surface modified method of MNPs isfeasible to gain probe-modified MNPs. The biotin-dUTP-labeled DNA fragments of gyrB genewas amplified by PCR, and the method of pseudomonas aeruginosa detection based on preparingMNPs has been developed in this work.

关键词： Magnetic macroparticles pseudomonas aeruginosa surface modification gyrB Chemiluminescence

作者: Yongjun Tang Zhiyang Li Liming Zhang Chao Ma Xiaolong Li Chuanyan Li Nongyue He

作者单位: State Key Laboratory of Bioelectronics,Southeast University,Nanjing 210096

会议类型: 国内会议

会议名称: 2012上海市研究生学术论坛暨第三届上海交通大学医(理)工研究生学术论坛

会议地点: 上海

会议语种:英文

页码: 1-9

在线出版日期: 2012-08-15（万方平台首次上网日期，不代表论文的发表时间）