Polyacrylonitrile-glycopolymer nanofibrous membranes for the BSA adsorption
Membranes are fast growing for bioseparationtechniques, however they are easy to be polluted by proteins andmany other components existed in the mixture. Carbohydratesare ubiquitous on the extracellular surface of living cells andmediate a myriad of biological recognition and signal processesand also can inhibit the adsorption of non-specific proteins.Glycopolymer nanofibrous membranes offer a new method tomimic cellular events. We describe a simple and effective methodof constructing glycosylated membrane surfaces using Poly (6-Ovinyladipoylglucose) (Poly-OVSEG) by a water phaseprecipitation homopolymerization process using thepolyacrylonitrile (PAN) as the supports. The structure wascharacterized by Fourier transform infrared spectroscopy (FTIR)and NMR. Composite membranes of PAN/poly-OVSEGnanofibers containing varying amounts (40–60％) of poly-OVSEG were subsequently prepared using electrospinning. Thenanofibers were studied by scanning electron microscopy (SEM).Bovine serum albumin (BSA) was used as probes to study thenonspecific interaction on this surface to proteins. The resultindicated that the novel glycopolymer nanofibrous membranes inthis work had well hydrophilicity and biocompatibility than PANmembranes which could prevent undesirable nonspecificadhesion of foreign proteins.
glyhomopolymer nanofibrous membranes BSAadsorption
Lei WANG Yan LI Cheng-cheng JIN Zhong-qing LIU Hua-li NIE Jing QUAN Li-min ZHU
College of Chemistry,Chemical Engineering and Biotechnology,Donghua UniversityShanghai,201620,P.R.Ch College of Chemistry,Chemical Engineering and Biotechnology,Donghua UniversityShanghai,201620,P.R.Ch
国内会议
2012上海市研究生学术论坛暨第三届上海交通大学医(理)工研究生学术论坛
上海
英文
1-4
2012-08-15（万方平台首次上网日期，不代表论文的发表时间）