Content determination of cocaine HCl in cocaine sample by HPLC
A simple and fast HPLC analysis method for cocaine HCl in cocaine sample was developed. In this work, cocaine in sample was separated using a Shim-pack VP-ODS column (250 mm × 4.6 mm, 5 μm) and a mobile phase of methanol-0.05％ TFA (35:65, v/v) at 35 ℃. The flow rate of a mobile phase was set to 1.0 mL/min and the UV detection was performed at 231 nm. Our results show that there was good linearity in the range of 1-1000 μg/mL of cocaine HCl concentration (R2=0.9998, n=6). The average recovery rate was 99.71％ (n=9, RSD=0.54％). The limit of detection was found to be ~0.05 μg/mL.
cocaine illegal drugs HPLC content determination
Zhen Guo Hui Zheng Yun Wei
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Be Institute of Forensic Science Ministry of Public Security P.R.C., Beijing 100038
国内会议
呼和浩特
英文
1-1
2011-07-20（万方平台首次上网日期，不代表论文的发表时间）