Online immobilized enzyme microreactor coupling to capillary electrophoresis for the glucose oxidase enzymatic hydrolysis
Compared with free enzymes, immobilized enzymes have improved stable high enzymatic efficiency and can be reused and separated easily. In this study, glucose oxidase (GOx) was immobilized in the inlet end of capillary as a plug and an online immobilized GOx capillary enzyme microreactor was developed. The enzymatic hydrolysis, electrophoretic separation and detection could be accomplished simultaneously via the prepared capillary, which combined the advantages of both enzyme microreactor and capillary electrophoresis. The present approach has potential applications in high-throughput screening of enzyme reactions and enzyme inhibitors.
Online immobilized enzyme microreactor Capillary electrophoresis GOx
Siming Wang Ping Su Yi Yang
College of Science, Beijing University of Chemical Technology, Beijing 100029, P.R. China
国内会议
呼和浩特
英文
2-2
2011-07-20（万方平台首次上网日期，不代表论文的发表时间）