Label-free Fluorescence Assay for Real-Time Monitoring of Site-Specific DNA Cleavage by EcoRI Endonuclease
In this paper, we demonstrated a fluorescent assay for lable-free, facile, and real-time detection DNA cleavage by EcoRI endonuclease using SYBR Green I (SGI) as signal probe. The fluorescence of SGI dramatically increased when the free SGI mixed with double-strands (dsDNA) substrate. While EcoRI was added into the mixture of dsDNA and SGI, the fluorescence of SGI decreased because of DNA cleavage by EcoRI. EcoRI–DNA interaction was monitored real-time based on the alteration of fluorescence signal before and after digestion with EcoRI. Compared with previously reported methods, this approach is selective, simple, convenient and cost-efficient without any labelling of the probe or of the target.
EcoRI endonuclease SYBR Green I label-free fluorescence oligonucleotides
Jing Deng Yan Jin
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and
国内会议
呼和浩特
英文
167-167
2011-07-20（万方平台首次上网日期，不代表论文的发表时间）