Label-Free Fluorescence Sensor for Hg2+ detection
In this paper, we have developed a label-free fluorescence method for sensitive detection of mercury ion in homogeneous solutions. The assay exploits the fluorometric signal change of hoechst33258 when DNA probe conformational transformed from a random coil to a hairpin structure upon binding to Hg2+. Under the optimum conditions, the system exhibits a dynamic response for Hg2+ from 10 nM to 2 μM with a detection limit of 1nM. It is a simple, selective, and sensitive method for Hg2+ detection.
Hoechst33258 Hg2+ fluorescence sensor oligonucleotide
Lin Wang Yan Jin
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and
国内会议
呼和浩特
英文
168-168
2011-07-20（万方平台首次上网日期，不代表论文的发表时间）