One-step Homogeneous Chemiluminescence Immunoassay based on Antibody-functionalized Gold Nanoparticles
One simple and sensitive chemiluminescence (CL) immunoassay system for antigens has been developed. The assay is based on the aggregation of gold nanoparticles (GNPs) that are coated with protein antigens in the presence of their corresponding antibodies. The aggregated GNPs can induce the stronger CL signal of luminol–H2O2. Under the optimized conditions, the CL intensity of the system was linear human IgG concentration in the range from 0.3 to 530 ng/mL. The new technique could be used for fast, high throughput detection of antigens in clinical diagnostic applications.
Immunoassay Chemiluminescence Gold nanoparticles Homogenous
Xiang Cui Yingying Qi Mei Liu Baoxin Li
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and
国内会议
呼和浩特
英文
227-227
2011-07-20（万方平台首次上网日期，不代表论文的发表时间）