Adsorption of cellulase on pretreated corncob at different pH

摘要：

Effective recycling of cellulase requires an in-depth understanding of cellulase adsorption and desorption.Here we studied the adsorption behaviors and stabilities of cellulase at different pH:acidic pH(<4.8)favored cellulase adsorption and alkaline pH(especially at pH 7 and 10)favored desorption;influence of pH on cellulase activity was reversible and the cellulase activity Can be retum to almost 100％by adjusting the pH to 4.8.Effect of pH,temperature on cellulase adsorption kinetics and isotherm were also explored in this study.At pH 4.8,temperature has no remarkable effect on the adsorption capacity of the cellulases onto substrate.At pH 7 and 10,higher pH and higher temperature favors cellulases desorption.Cellulase adsorption only at pH 4.8 Can provided a good fit(R2>0.96)of the simple pseudo-first-order kinetic model and Langmuir adsorption isotherm models (R2>0.99).

关键词： Cellulase adsorption Cellulase desorption Enzyme recycling pH adjustment Langmuir adsorption isotherm

作者: Ruoyu Du Rongxin Su Wei Qi Zhimin He

会议类型: 国内会议

会议名称: 天津大学2011年博士生论坛化工分论坛

会议地点: 天津

会议语种:英文

页码: 6-21

在线出版日期: 2011-08-19（万方平台首次上网日期，不代表论文的发表时间）