Hydrogen sulfide and cystathionine-γ-lyase promote acute lung injury induced by acute pancreatitis in rats

摘要：

Acute pancreatitis(AP)is well-known to induce the injuries to extrapancreafic organs.Because respiratorydysfunction is the main cause of death in patients with severe AP,acute pancreatitis-associated lung injury(A-PALI)is a great challenge for the clinicians.This study aimed to investigate the potential role of H2S in thepathogenesis of APALI.54 SD rats were radomly divided into three groups：AP group made by injection of sodiumdeoxycholate into the common bile duct,control group made by sham operation’and treatment group made by in—trapefitoneal injection of propargylglycine(PAG.CSE inhibitor)into AP rats.Histopahtology of the lung Was ex—amined and the exDrcssion of CSE and TNF—α mRNA in lung tissue Was detected by RT-PCR.H2S level in thescmm WLq detected spectrophotometrically.The results showed that the serum concentration of H2S and CSE andTNF-α expression in the lung were increased in AP rats.Intraperitoneal injection of PAG could reduce theserum concentration of H2S,reduce CSE and TNF-α expression,and elleviate the lung pathology.Taken togeth-er.our findings suggest that H2S/CSE system is crucially involved in the pathological process of APALI and rep-resents a novel target for the therapy of APALI.Acute pancreatitis associated lung injury;H2S;cystathionine-γ-lyase;propargylglycine;TNF-α

作者: Zhen Qu YongJiang FengZhu Zhen Li Sun Bao QiangWu Guang Hua Luo Bo Hua Huang

会议类型: 国内会议

会议名称: 2012国际肝胆胰外科昆山论坛暨第24届肝胆胰外科学术经验交流会

会议地点: 江苏昆山

会议语种:英文

页码: 259-263

在线出版日期: 2012-05-25（万方平台首次上网日期，不代表论文的发表时间）