Combined Transfection of the Three Transcriptional Factors, PDX-1, NeuronD1, and MafA Causes Differentiation of Bone Marrow Mesenchymal Stem Cells Into InsuHn—Producing Cells
Aims：The goal of cell transcription for treatment of diabetes is to generate surrogateβ-cells from an appro—pilate cell line.However,the induced replacement cells have showed less physiological function in producing in—sulin compared with normal β-cells.Methods：Here,we report a procedure for induction of insulin-producing.cells(IPCs)from bone ma/Tow murine mesenehymal stem cells(BM-mMSCs).These BM-mMSCs have the poten—tial to differentiate into insulin—producing cells when a combination of PDX-1(pancreatic and duodenal home—obox—1),NeuroDl(neurogenic differentiation-1)and MafA(V-maf musculoaponeurotic fibrosarcoma oncogenehomogog A)genes are transfected into them and expressed in these cells。Results：Insulin biosynthesis and seere—tion were induced in mMSCs into which these three genes have been transfected and expressed。The amount ofinduced insulin in the mMSCs which have been tranfected with the three genes together is significantly higherthan in those mMSCs that were only transfected with one or two of these three genes.Transplantation of the trans—feeted cells into mice with streptozotoein—induced diabetes results in insulin expression and the reversal of theglucose challenge.Conclusions：These findings suggest major implications for cell replacement strategies in gen—eration of surrogate β-cells for the treatment of diabetes.
Guo Qingsong Zhu Mingyan Wang Lei Fan Xiangjun Lu Yuhua Wang Zhiwei Zhu Shoun Wang Yao Huang Yan
Department of General Surgery，Affiliated Hospital of Nantong University，Nantong 22600 1，China Depart Department of General Surgery，Affiliated Hospital of Nantong University，Nantong 22600 1，China Depart Department of General Surgery，Affiliated Hospital of Nantong University，Nantong 22600 1，China Depart
国内会议
2012国际肝胆胰外科昆山论坛暨第24届肝胆胰外科学术经验交流会
江苏昆山
英文
313
2012-05-25（万方平台首次上网日期，不代表论文的发表时间）