A set of proteins interacting with papaya ringspot virus (PRSV) Nia-Pro protein identified in a two-hybrid screening
The protein-protein interactions between viral proteins and host factors have crucial roles in plant virus infection and host defense. The potyviral nuclear inclusion protein a protease (Nia-Pro) is involved in various steps of viral infection. In this study,we constructed a Carica papaya L. Plant cDNA library to investigate the host proteins interacting with Papaya ringspot virus (PRSV) Nia-Pro using a Sos recruitment two-hybrid system (SRS). We confirmed that the full-length EIF3G , FBPA1, FK.506BP, GTPBP , MSRB1 and MTL from papaya can interact specifically with PRSV Nia-Pro in yeast,resepctively. These proteins play important roles in plant proteins translation, biotic and abiotic stress, energy metabolism and signal transduction. The possible functions of the interactions between these host proteins and Nia-Pro in PRSV infection and host defense are discussed.
Sos recruitment two-hybrid system papaya ringspot virus Nia-Pro protein-protein interaction
L. GAO W.T.SHEN P. YAN X.Y. Li P. ZHOU
College of Agriculture, Hainan University, Haikou 570228, Hainan, People”s Republic of China Key Bio Key Biotechnology Laboratory for Tropical Crops, Ministry of Agriculture, Institute of Tropical Bios Key Biotechnology Laboratory for Tropical Crops, Ministry of Agriculture, Institute of Tropical Bios
国内会议
2011年度中国热带作物学会遗传育种专业委员会年会暨学术研讨会
南昌
英文
1-5
2011-08-01（万方平台首次上网日期，不代表论文的发表时间）