Cloning and Sequence analysis of two banana bunchy top virus genome in Hainan
TAS-ELISA detection samples which was infected banana bunchy top virus (BBTV), cloning two genome of BBTV HaiKou 2 and BBTV HaiKou 4 isolates of its total DNA template of tender pseudostem and leaf by Reverse-PCR method. The result shows, the full-long sequence of HaiKou 2 DNA 1-6, Satellite DNA are 1103, 1067, 1058, 1041, 1013, 1082 and 1093 nt, respectively, HaiKou 4 DNA1-6 full-long sequence as 1103, 1055, 1059, 1041, 1013 and 1081 nt, respectively. Each component of two isolates non-coding region including one high homologous CR-SL and CR-M; Coding region has a open reading frame (ORF), and a small ORF existence inside DNA1 large ORF. Sequence analysis shows, DNA1 component of two isolates comparing of others are the most conservative. DNA2 component are maximum variation. According to Karan classification, we show that HaiKou 2 and HaiKou 4 isolates are the member of Asian group. In addition, HaiKou 2 isolate exists Satellite DNA, coding one of 33.1kD replication initiation protein, while HaiKou 4 isolate was does not existence.
banana bunchy top virus genome cloning sequencing
Nai-tong Yu Yu-liang Zhang Jian-hua Wang Wen-jun Yang Zhan-song Lin Zhi-xin Liu
Institute of Tropical Bioscience and Biotechnology, CATAS 4 Xueyuan Road, Haikou 571101, China Colle Institute of Tropical Bioscience and Biotechnology, CATAS 4 Xueyuan Road, Haikou 571101, China Institute of Tropical Bioscience and Biotechnology, CATAS 4 Xueyuan Road, Haikou 571101, China Insti
国内会议
2011年度中国热带作物学会遗传育种专业委员会年会暨学术研讨会
南昌
英文
125-136
2011-08-01（万方平台首次上网日期，不代表论文的发表时间）