Isolation and characterization of resistance gene analogs from mango, evidence for high variation range in NBS-LRR gene family of mango
Nucleotide-binding site (NBS) and leucine-rich repeat (LRR) class gene can be obtained from plant genome based on PCR-based strategy according to conserved motif of NBS domain. In order to clone R class gene responsible for binding pathogen virulence factors in mango, we designed six pairs of degenerate primers; the left primer corresponding to protein sequence of P-loop of NBS domain and the right primer from kinase 2 of NBS domain. Twenty three clones were isolated from the PCR fragment and then sequenced. Their homologous extent between RGAs varied from 33.3％ to 98.5％. Among them, sixteen DNA sequence can be translated into amino sequence. The high homolog in protein sequence of P-loop and kinase 2 of NBS domain between RGAs isolated in the study and characterized R gene indicate these cloned sequence belong to the NBS-LRR gene family. Moreover, these RGAs can be classified into non-TIR-NBS-LRR gene family because of only tryptophan (w) occurs in the last residual of kinase-2 of all RGAs isolated in the investigation. From our result, we can conclude that R NBS-LRR genes possessed a high variation range in mango genome, which is advantage for mango to recognize different pathogen virulence factors.
雷新涛 黄丽芳 姚全胜 肖勇 曹红星
中国热带农业科学院椰子研究所，海南文昌文清大道496号 571339
国内会议
2011年度中国热带作物学会遗传育种专业委员会年会暨学术研讨会
南昌
英文
137-143
2011-08-01（万方平台首次上网日期，不代表论文的发表时间）