Human umbilical cord-derived mesenchymal stem cells differentiate into epidermal-like cells using a novel co-culture technique
Human umbilical cord-derived mesenchymal stem cells(hUCMSCs)isolated from human umbilical Wharton”s Jelly are a population of primitive and pluripotent cells．In different specific conditions，they can differentiate into different cells，such as adipocytes，osteoblasts，chondrocytes，neurocytes，endothelial cells etc．However,few reports on differentiating hUCMSCs into epidermal cells in vitro were published.There are several methods of MSCs differentiation for choice，such as the specific induction medium，direct or indirect co-culture. In this study，We have developed a microporous membrane-based indirect co-culture system forhUCMSCs differentiation，in which epidermal stem cells were seeded on the bottom of microporous membraneand the culture flask of the 6-well dish,hUCMSCs were seeded on the top of microporous membrane．This system allows real-time conditioning of the culture medium with human epidermal cells while still maintaining the complete separation of the two cen types．HUCMSCs in this indirect co-culture system expressed the early markers of epidermal cell lineage,P63，cytokcratin19(CK19)，and β1-integrin．Their morphologies were changed from spindle-like fibroblastic appearances to oblate or irregular shapes under phase contrast microscopy.All these data not only highlight the possibility ofin vitro control of hUCMSCs into epidermal lineage，but also suggest a novel indirect co-culture model for MSCs differentiation．
human umbilical cord-derived mesenchymal stem cells differentiation epidermal-like cells microporous membrane indirect co-culture system
Li Dong-jie Chai Jia-ke Han Yan-fu Sun Tian-jun
Burn Institute,the First Affiliated Hospital of PLA General Hospital，Beijing,China
国内会议
西安
英文
199-208
2011-06-01（万方平台首次上网日期，不代表论文的发表时间）