Baicalin prompts migration of neural progenitor cell by stimulating hypoxia-induced astrocyte in vitro.
The migration of transplanted neural progenitor cell (NPC) or endogenesis NPC to damagedsections in the central nervous system (CNS) is a critical step for NPC therapy, A combination of extracellular signals and microenvironmental conditions is necessary for the migration of NPC. Astrocytes, as the major nonneuronal cells in the brain, can be activated by injuries to express chemoattractants that can direct the migration of NPC. Data in this paper has firstly shown baicalin can increase the chemotactic ability of astrocytes on NPC under hypoxia condition in vitro by upregulating of VEGF and MCP-1 in astrocytes. Astrocytes treated with 160μg/ml baicalin and exposed to hypoxia forl8h at the same time. Migration assays with transwell showed the conditioned medium of hypoxia and balcalin-induced astrocytes increased the migration of NPCs compared to baicalin absence. Real-time RT-PCR assay indicated that mRNA of VEGF and MCP-1 has been upregulated by baicalin in astrocytes exposed to hypoxia and block chemotaxis assay furthermore demonstrated that the high expressions of VEGF and MCP-1 involved in the improvement of the migration of NPCs. These results provided supports for exploring a clinical role for baicalin in NPC therapy.
neural progenitor cell migration astrocyte hypoxia baicalin VEGF MCP-1
徐强 姜西娟 王少侠 赵亚莉 石田寅夫
2nd hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China Tianjin University of Traditional Chinese Medicine, Tianjin, China Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka, Japan
国内会议
天津
英文
103-110
2010-07-01（万方平台首次上网日期，不代表论文的发表时间）