Molecular Cloning and Expression Analysis of 13 MADS-Box genes in Betula platyphylla
MADS-box genes encode a family of transcription factors that control a diverse range of processes in flowering plants. Gibberellins (Gas) are required for normal growth of plant organs through the promotion of cell division and/or cell elongation. In addition, Gas promote certain developmental switches or phase changes, including seed germination, the juvenile to adult transition, and the transition from vegetative to reproductive development in some species. Birch (Betula platyphylla) is one of the most economically important forest trees in the boreal region of China. Naturally occurring birch trees are extremely large and long-lived trees with a juvenile period of 10 to 15 years, although this is shortened to 2 to 3 years in fortified breeding conditions. Therefore, the two-year-old birch seedlings investigated in this study represent the period of the juvenile to adult transition.In this study, 13 unique MADS genes were cloned from Betula platyphylla Suk., in which 8 MADS genes were new genes, and 2-year old betula seedlings propagated in glasshouses were selected as plant materials. The expression profile of each BpMADS was investigated during the growth season and following GA treatments by real-time quantitative reverse transcription polymerase chain reaction.
Betula platyphylla Suk MADS-box genes flowering gibberellins
Li Hui-yu Liu Fei-fei Wang Shan Liu Gui-feng
State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road,Harbin 150040, China
国内会议
乌鲁木齐
英文
11-12
2011-07-01（万方平台首次上网日期，不代表论文的发表时间）