Real-Time Quantitative Polymerase Chain Reaction With SYBR Green I Detection for Estimating Copy Numbers of Porcine Endogenous Retrovirus From Chinese Miniature Pigs
Porcine endogenous retrovirus (PERV) in the pig genome represents a potential infectious risk in xenotransplantation. Chinese miniature pigs have been considered to be potential organ donors in China. However, an adequate level of information on PERV from Chinese miniature pigs has not been available. We established an SYBR Green I-based real-time quantitative polymerase chain reaction (PCR) assay for estimating copy numbers of PERV integrated in the host genome. The assay was 100-fold more sensitive compared with conventional PCR. We also evaluated the specificity and reproducibility of the assay. We statistically analyzed the difference in PERV copy numbers integrated into the genomes of Wuzhishan pigs versus Bama minipigs. This approach will be useful to screen donor pigs as well as to examine clinical samples from human subjects treated with porcine xenotransplantation products for evidence of PERV transmission.
Y. Ma Y. Yang M. Lv Q. Yan L. Zheng F. Ding J. Wu K. Tian J. Zhang
Laboratory for Viral Safety of National Centre of Biomedical Analysis Laboratory for Viral Safety of National Centre of Biomedical Analysis Institute of Transfusion Medic Laboratory for Viral Safety of National Centre of Biomedical Analysis Nanning,Guangxi Province,china
国内会议
2011东莞第二届国际小型猪学术论坛暨大型实验动物生物医药研究应用研讨会
东莞
英文
379-382
1900-01-01（万方平台首次上网日期，不代表论文的发表时间）