A method of quality monitoring for fresh milk—Flow cytometric analysis for somatic cell in milk
In order to monitor change of T lymphocyte subpopulation in milk derived from infected bovine mammary glands. 30 lactating cows with mastitis were compared to 10 normal controls. The results showed that CD4+ T cells and CD8+ T cells were significantly higher in milk derived from infected bovine mammary glands compared to that of normal controls(P<0.0001). IL-6, IL-1 and IFN-γ were found in normal and infected milk samples;IL-1a( 26/30), IL-1β( 28/30),IL-12 (29/30) and TNF-a (30/30) were only found in infected milk samples;Neither IL-2 nor IL-4 was found in any milk samples. In conclussion, we can monitor quality of fresh milk by analysis of CD4+ T cells and CD8+ T cells in milk using flow cytometric.
Milk Quality monitoring Flow cytometric analysis CD4+ T cells CD8+T cells
Haiyan Wang Zhiguo Zhao Weihua Ao Linli Zhao Gang Li Ying Chen Guoqing Pan Jie Bao
Inner Mongolia Entry-Exit Inspection and Quarantine Bureau. No. 12, Erdos Street, Huhhot, Inner Mong Institute of Food Safety, Chinese Academy of Inspection and Quarantine. No.3, Gaobeidian North Road,
国内会议
青岛
英文
14-22
2011-10-19（万方平台首次上网日期，不代表论文的发表时间）