Application of Surface Fitting Model for Estimation of the Number of Vibrio parahaemolyticus in Seafood based on Enrichment and Real-Time PCR
The rapid, accurate and convenient enumeration of Vibrio parahaemolyticus in seafood is helpful for safety of seafood. However, the present methods of enumeration of V. parahaemolyticus are either insensitive or laborious. We employed an enrichment process which could enhance detection limit to develop a predictive model for estimation of the initial cells of V. parahaemolyticus in seafood. First, Response Surface Design was used to acquire evenly distributed enrichment combinations which helped to result in correctly fitted function and proper predition bounds. Second, sterile samples of seafood were inoculated in alkaline saline peptone water according to the enrichment combinations and incubated, and subsamples of 6-h and 8-h enrichment were detected by real-time PCR. Last, the cycle threshold (Ct) values for subsamples of 6 h and 8 h of incubation were regarded as 2 independent variables separately and the initial concentration of V. parahaemolyticus were regarded as a dependent variable to develop a surface fitting model by MATLAB software. And the 95％ prediction bounds of this fitted surface was also shown. After detection of 30 additional artificially contaminated samples by this method of predictive model, this surface fitting model was proven to be quite useful in the enumeration of V. parahaemolyticus in seafood.
JunTao Jia Jian Zhang ZhengYi Li YingHui Jiang WeiXing Ma Jing Tang YingAn Jiang
Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, P. R. China Ocean University of China, 266003, P. R. China
国内会议
青岛
英文
148-155
2011-10-19（万方平台首次上网日期，不代表论文的发表时间）