Validation of Collaborative Trial of Event-Specific Qualitative PCR Detection Method of Genetically Modified RT73 Rapeseed
The qualitative event-specific PCR detection method of genetically modified (GM) RT73 rapeseed was developed based on the cloned 3” end flanking sequence of RT73 rapeseed integration before the collaborative trial. The specificity of the method for GM RT73 rapeseed was validated employing several different GM rapeseed lines, GM maize lines, GM soybean line, non-GM rapeseed, and other non-GM crops. In this collaborative study, the developed method has been validated through a collaborative study by twelve laboratories from six countries. The sensitivity of this method was evaluated using several mixed rapeseed meals with different GM RT73 rapeseed contents from 5.0％ to 0.01％ prepared by our laboratory. The evaluated results showed that all the rapeseed endogenous reference high mobility group protein gene (HMG I/Y), figwort mosaic virus 35S (FMV 35S) promoter and RT73 event-specific fragment could be detected from the sample with 0.1％ (w/w) rapeseed at a satisfaction level of more than 95％. All results from the twelve laboratories indicated that the developed method could be considered fit for the detection and identification of GM RT73 rapeseed.
Liangwen Pan Sbuya Zhang Litao Yang Hermann Broil Fenghua Tian Dabing Zhang
GMO Detection Laboratory, Shanghai Entry-Exit Inspection & Quarantine Bureau, 1208 Minsheng Road, Sh School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shangh Federal Institute for Risk Assessment, Thielallee 88-92,14195 Berlin, Germany Bio-Engineer College, East China University of Science and Technology, 130 Meilong Road, Shanghai 20
国内会议
青岛
英文
169-175
2011-10-19（万方平台首次上网日期，不代表论文的发表时间）