Determination of 15 mycotoxins in foods and feeds using high performance liquid chromatography-tandem massspectrometry with gel permeation chromatography combined QuEChERS purification
A reliable, sensitive and selective method was successfully developed to determine 15 different mycotoxins simultaneously in foods and feeds. In this method, the homogenized sample was first treated with gel permeation chromatography <GPC) to eliminate most of coextracts, such as pigments, lipids and waxes. Modified QuEChERS method was then carried out as a further cleaned up step, during which the polar interfering compounds such as organic acids and sugars were removed by vortexing with ODS in acetonitrile phase. Using double sample injection method, the analytes were separated by ZORBAX Eclipse Plus C18 (1.8um, 2.1×100mm) and detected by a MS/MS system with electrospray ionization (ESI) at muti-reaction monitoring (MRM) mode. The result indicated that the LOD of 15 mycotxoins were ranged from 0.07～5.0 μg/kg. Meanwhile, high correlation coefficients (r2>0.996) of 15 mycotoxins were obtained within their respective linear ranges. The average recoveries for lower, intermediate, and high spiked levels ranged from 80.1％～95.5％ in company with RSD ranged from 10.5％～19.6％. The method not only represents many advantages including simple pre-treatment, good purification and high sensitivity, but also successfully fit the minimum limiting level requests from various countries including EU USA and Japan.
HPLC-MS-MS GPC QuEChERS mycotoxins foods and feeds
Xiaoming Gong Hongbing Wang Yibing Zhang Jing Dong Jun Sun Jian Li Jinling Yu Kai Li
Weifang Entry-Exit Inspection and Quarantine Bureau, Weifang, Shandong, P.R. China Yellow Sea Fisheries Research Institute, Qingdao, Shandong, P. R. China
国内会议
青岛
英文
407-415
2011-10-19（万方平台首次上网日期，不代表论文的发表时间）