Determination of valnemulin in swine tissues by high-performance liquid chromatography-tandem mass spectrometry
A simple, sensitive and reliable method for the determination of valnemulin (VLM) in swine muscle, kidney and liver by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. The analyte was extracted using the mixture of acetonitrile and 0.01mol·L-1 hydrochloric acid. The crude extracts were subjected to liquid-liquid partition with w-hexane followed by solid phase extraction using a mixed-mode strong cation-exchange column. The chromatographic separation was performed using a gradient on a Waters Symmetry Shield RP C18 column with a mobile phase of acetonitrile-water containing 0.1％ formic acid, and the analyte was detected by multiple reaction monitoring (MRM) using positive eletrospray ionization (ESI). The method has been validated at the limit of quantification (LOQ), at half of the MRL and at the MRL. The limit of detection (LOD) and LOQ of swine tissues were 1 ng·g-1 and 5 ng·g-1 respectively. The mean recoveries from fortified samples ranged from 84.2％ to 92.3％, with coefficients of variation ranging between 7.5％ and 15％.
valnemulin muscle kidney liver LC-MS/MS
Qiushi Huang Jiancheng Li Haiyang Jiang Xia Xi Lijun Xia Jianzhong Shen Shuangyang Ding
Huangdao Entry-Exit Inspection and Quarantine Bureau, Qingdao 266500, China Department of Pharmacolo Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural Univer Department of Pharmacology and Toxicology, College of Veterinary Medicine, ChinaAgricultural Univers
国内会议
青岛
英文
59-64
2011-10-19（万方平台首次上网日期，不代表论文的发表时间）