Simultaneous Determination of 44 Pesticides in Tobacco Applying a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) Procedure and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
A novel, simple and rapid method has been developed to screen for the presence of 44 pesticide residues in tobacco. The method uses a new sample preparation procedure loosely based on QuEChERS (Quick, Easy,Cheap, Effective, Rugged and Safe) methodology. The pesticides are extracted with acetonitrile and purified via a mixed sorbents of primary secondary amine (PSA), octadecyl silane (C18) and graphitized carbon black (GCB). Analysis is performed by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). AH pesticides are monitored in the positive or negative ionization mode in a single run due to the fast polarity switching capability of the mass spectrometer. Two precursor-product ion transitions are monitored for each compound in the multi-reaction monitoring (MRM) mode. Quantification is carried out using matrix-matched standards calibration. The method has been validated for the tobacco types of Burley, Oriental and Virginia from different countries. Recoveries of the proposed method for the spiked samples ranged from 71.1％ to 109.8％ and relative standard deviations (RSD) are below 10％. The limits of quantification (LOQ) are all below the guidance residue levels proposed by the Agrochemical Advisory Committee of CORESTA.
Pesticides Tobacco QuEChERS UPLC-MS/MS
CHEN XUYAN GE BAOKUN ZHAO KONGXIANG
Tianjin Entry & Exit Inspection and Quarantine Bureau, Tianjin 300461,China
国内会议
青岛
英文
65-72
2011-10-19（万方平台首次上网日期，不代表论文的发表时间）