Overexpression of a Mains vacuolar Na+/H+ antiporter gene ”MdNHXl) enhances its drought tolerence in apple rootstock M.26
Drought is a major abiotic factor limiting plant growth and crop production. To generate apple adapting to arid soil, we had transformed apple rootstock M.26 with MdNHXl, a vacuolar Na+/H+ antiporter gene from Luo-2, a salt-tolerant rootstock of apple (Malus domestica Borkh.) We report here that overexpression of the MdNHXl gene confers enhanced drought tolerance to the transformed apple rootstock M.26.The transgenic lines and the wild-type plants were treated for short-term natural drought stress for 4 days, and then rehydrated. Photosynthetic characteristics, relative leaf water content (RWC), cell membrane damage, root activity were monitored. Compared with the wild-type plants, transgenic lines performed better as the follow 3 aspects.(l) MdNHXl transgenic M.26 protects photosynthetic machinery in transgenic lines(Fig. 1). (2) MdNHXl transgenic M.26 retains more water during drought stress than wild-type(Fig. 2). (3) MdNHXl transgenic M.26 maintains the stability cell membrane damage than wild-type. These results suggest that the overexpression of vacuolar Na+/H+ antiporter gene could increase the drought tolerance of apple rootstock M.26.
Na+/H+ antiporter gene Drought tolerance Apple rootstock Transgenic M.26
LI Chao WEI Zhi-wei Liang Dong LIU Chang-hai WANG Rong-chao MA Feng-wang
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China
国内会议
海口
英文
22-23
1900-01-01（万方平台首次上网日期，不代表论文的发表时间）