The experimental and clinical study of IL-1β, A2M, Cathepsin D affecting deep venous thrombosis
OBJECTIVE (1)To screen the distinguishing patterns of protein and gene expression of femoral vein vascular tissue at different status (status of pre-thromgenesis, thrombogenesis peak, and non-thrombogenesis), by establishing a rat traumatic deep vein thrombosis model. (2) To formulate uniform clinical standards for diagnosis、inclusion and exclusion in patients, collect DVT patient”s venous blood, and screen differentially proteins expression in 2 groups (the human thrombosis group/non-thrombosis group). (3) Comparing the expression of the protein and gene in rats” venous vascular tissue and in human”s venous blood, in order to find out characteristic protein expression (including IL-1|3, IL-6, A2M, Cathepsin D and Cathepsin G) in rats” vein vascular tissue, which were highly relevant to the endothelium and platelet, (4) Using ELISA to further identify the different changes of expression about these important proteins in human blood, and explore the potential role of these proteins in the process of endothelial cell injury, platelet activation, inflammatory factor activation, regulation of the interaction mechanism, and the function of promoting thrombosis.
Liqing Yao
Rehabilitation Department of Second Affiliate Hospital of Kunming Medical University
国内会议
昆明
英文
186-187
2011-09-16（万方平台首次上网日期，不代表论文的发表时间）