6-Shogaol decreased LOX-1 expression, inhibition of oxidative stress and attenuates endothelial cell apoptosis
Backgroud and purpose：6-shogaol is the major bioactive compounds present inZingiber officinale, and possesses the anti-atherosclerotic effect. Here,we evaluatedthe effects of 6-shogaol on oxLDL-induced human umbilical vein endothelialcells(HUVECs) injuries and its possible molecular mechanisms.Experimental approach： human umbilical vein endothelial cells(HUVECs) waspre-incubated with or without 6-Shogaol and stimulated by oxidized low densitylipoprotein (ox-LDL) in the presence or absence of LOX-1 siRNA or caspase-3inhibitor.Cell viability, cell apoptosis，cellular Reactive Oxygen species, NADPHoxidase and caspase-3, 8, 9 activities were measured.Key results：6-shogaol decreased the oxidation of LDL into oxLDL and attenuated theoxLDL-rcaused oxidative stress and cell damage. Exposure of HUVECs to 200 ug/mloxLDL induced masive cell apoptosis, however, 6-shogaol defended against suchinjuries. Application of Lox-1 small interference (si)RNA into HUVECs reduced thetranslation of this membrane receptor, and simultaneously increased 6-shogaolprotection from oxLDL-induced cell apoptosis. By comparison, overexpression ofLox-1 attenuated 6-shogaol protection. 6-shogaol inhibited oxLDL-induced Lox-1mRNA and protein expressions. Both 6-shogaol and Lox-1 siRNA decreasedactivities of caspases-9，-3, but not caspases-8. Pretreatment with caspase-3 inhibitorDEVD一CHO (25 umol/L) synergistically promoted 6-Shogaol”s protection againstcell apoptosis.Conclusions andimplications：this study shows that 6-shogaol can protect HUVECsfrom oxLDL-induced apoptotic insults via downregulating Lox-1-mediated activationof the caspase protease pathway.
姜烯酚 脐静脉内皮细胞 氧化应激 动脉粥样硬化
Yun kai Wang Jun Bo Ge Haozhu Chen XiaoliangLeon Xu Ya Jun Hong Dai Jing Wei Mao Wu Ye Zhao Quan Huang Hong Mao Qun wei Cheng Rui Zhen Chen
Department of Cardiology, The First Affiliated Hospital of Zhejiang Chinese TraditionalMedical Unive Shanghai Institute of Cardiovascular diseases, Zhongshan Hospital, Fudan University, Shanghai200032, Department of Pathology, Sloan Kettering Institute, Memorial Sloan Ketter ing Cancer Center,New York Department of Cardiology, The Second Affiliated Hospital of Zhejiang University
国内会议
广州
英文
1-2
2011-11-25（万方平台首次上网日期，不代表论文的发表时间）