Paeoniflorin inhibits vascular inflammtion, attenuates vascular oxidative stress, and improves endothelial function in high cholesterol diet fed rabbit
Objective:Paeoniflorin has antioxidative, antiinflammatory properties. In this study, weexamined its effectiveness against vascular oxidative stress, inflammation, andendothelial dysfunction in accelerated atherosclerosis.Methods:New Zealand white rabbits received an infusion of Paeoniflorin, after 6weeks high-cholesterol chow. Human umnilical vein endothelial cells (HCAECs)were incubated with Paeoniflorin before stimulation with oxLDL.Results:Paeoniflorin(2.5mg,5mg/kg) attenuated vascular oxidative stress significantlyand improved Endothelium-dependent relaxation(EDR).Paeoniflorin markedlyreduced vascular upregulation of ICAM-1 expression，plasma sP-selectin and ET-1concentrations；augmented vascular endothelial NO synthase activity, cyclic GMP(cGMP) content and plasma NO content (P<0.05 versus Control rabbits). In culturedendothelial cells, Paeoniflorin(5, 25, 50uM) increased eNOS phosphorylation atSer1177, leading to an increased production of nitric oxide. Furthermore, Paeoniflorinattenuated oxLDL induced generation of reactive oxygen species, caspase-3 activityand cellular apoptosis.Conclusion:Paeoniflorin protects against endothelial injury and enhances theendothelium-dependent vasodilatation, which is mediated in part through theactivation of eNOS, the inhibition of inflammation, the attenuation of oxidativestress and the decrease of endothelial cell apoptosis. Paeoniflorinor and its derivativesmay be useful for the treatment and/or prevention of atherosclerosis.
芍药甙 动脉粥样硬化 抗氧化机制 中药治疗
Yun kai Wang Xiaoliang Leon Xu HaozhuChen Ya Jun Hong Wei Mao Dai Jing Ming Zhu Zhao Quan Huang Wu Ye Rui zhen Cheng Junbo Ge
Department of Cardiology, The First Affiliated Hospital of Zhejiang Chinese Traditional Medical Univ Department of Pathology, Sloan Kettering Institute, Memorial Sloan KetteringCancer Center,New York, Shanghai Institute of Cardiovascular diseases, Zhongshan Hospital, Fudan University, Shanghai, 20003 Department of Cardiology, The Second Affiliated Hospital of Zhejiang University Department of Cardiology, The First Affiliated Hospital of Traditional Medical University Shanghai Institute of Cardiovascular diseases, Zhongshan Hospital, FudanUniversity, Shanghai, 200032