Comparative genomic analysis of five pairs of virulent parental/attenuated vaccine strains of PRRSV
Porcine reproductive and respiratory syndrome virus (PRRSV), the causative agent of porcine reproductive and respiratory syndrome, is responsible for serious disease in pigs resulting in substantial economic losses in the porcine industry. An attenuated vaccinestrain, HuN4-F112, was obtained by passaging virulent PRRSV strain HuN4 on Marc-145cells (for 112 passages), and the full-genomic sequence was determined. To understandthe molecular basis of attenuation of PRRSV, we compared and analyzed the genomic sequences of HuN4/HuN4-F112, together with those of other four virulent parental/attenuated vaccine strains. Among the 19 PRRSV proteins, two (NSP6 and NSPB) werehighly conserved, without any mutations and considered irrelative to attenuation. The mutation rates of envelope-associated structural proteins were obviously higher than those of most non-structural proteins. It is interesting that the gene of the smallests tructural protein, E protein, had the highest mutation rate among all of the structural genes analyzed, and also harbored a highly variable region. Our results indicate that determinants of PRRSV attenuation are multigenic products of both non-structural and structural genes. To our knowledge, this is the first report showing that the envelope-associated structural proteins (including E and GP2-GP5 proteins) may play a significant role. These findings contribute towards our understanding of PRRSV attenuation and will provide an important clue for further study.
猪繁殖与呼吸综合征病毒 HuN4-F112 细胞免疫
Tong-Qing An Zhi-Jun Tian Yan-Jun Zhou Yan Xiao Jin-Mei Peng Jin Chen Yi-Feng Jiang Xiao-Fang Hao Guang-Zhi Tong
Division of Swine Infectious Diseases,National Key Laboratory of Veterinary Biotechnology,Harbin Vet Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,Chin Division of Swine Infectious Diseases,National Key Laboratory of Veterinary Biotechnology,Harbin Vet
国内会议
上海
英文
1-6
2011-10-01（万方平台首次上网日期，不代表论文的发表时间）