Prime-boost immunization with HA/C3d DNA followed by a recombinant pseudorabies virus boost enhanced protective immunity against H3N2 swine influenza virus in mice
DNA and recombinant virus vaccines against swine influenza virus (SIV) have been pursued with promising results, but induce poor immunogenicity. This study evaluated the effects of a vaccine regimen inmice including priming with three DNA vaccines expressing soluble HA (sHA), complete HA (tmHA), orsHA fused with three copies murine C3d (sHA-mC3d3) and boosting with recombinant pseudorabiesvirus expressing HA (rPRV-HA). Immune responses were monitored by ELISA, HI assays, and virus neutralization. Protective efficacy was evaluated by virus isolation from lungs, distribution in tissues, and pathology following challenge with H3N2 SIV. Priming with sHA-mC3d3 and boosting with rPRV-HAinduced higher levels of HA-specific antibodies and yielded the most effective protection. This findingimplied that priming with a DNA vaccine expressing C3d fused with antigen and boosting with a recombinant vector vaccine is an effective way to induce protective bumoral immunity and prevent some infectious diseases.
DNA重组 蛋白免疫 流感病毒 病毒分离
Guo-Xin Li Yan-Jun Zhou Hai Yu Zhi-Jun Tian Li-Ping Yan Qiang Zhang Shou-Ping Hu Guang-Zhi Tong
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin V Technical Center for Animals, Plants and Foods Inspection and Quarantine Shanghai Entry-Exit Inspect
国内会议
上海
英文
1-7
2011-10-01（万方平台首次上网日期，不代表论文的发表时间）