Multiplexed quantum dots-IHC to study the signalin
Objective To determine the signaling axis of β2-M-pCREB-AR and the expression of RANKL inPCa and bone stromal cells in clinical primary and bone metastatic PCa (PCa-met) tissuespecimens, by using multiplexed quantum dots immunohistochemistry (mQD-IHC).Materials and Methods To establish the feasibility of mQD-IHC staining protocol (Xing, et al.Nature Protocol, 2007), we focused our analysis on primary and bone metastatic clinical PCatissue specimens at the single cell level. We compared the expression of β2-M, pCREB, and AR inbenign, prostatic intraepithelial neoplasia (PIN), and cancerous regions of the primary PCa andRANKL expression in bone PCa-met. The status of EMT in the bone PGa-met cells wasdetermined by the expression of an epithelial cell adhesion protein (EpCAM) and vimentin(VM).
免疫组化 信号转导通路 原发性骨转移 前列腺癌 微球蛋白
Guodong Zhu Hui He Dalin He
Department of Urology,The First Affiliated Hospital of Medical School,Xian Jiaotong University
国内会议
福州
英文
206-207
2011-09-16（万方平台首次上网日期，不代表论文的发表时间）