TLR4 signaling promotes tumor development in gastric cancer
Chronic infection and inflammation have been regarded as one of main course of carcinogenesis and development.Toll-like receptors(TLR)expressed on inflammatory cells play a key role in host defense against pathogens，benefiting the host.Also，recent studies showed that TLRs were also expressed by tumor cells.However, the mechanisms by which TLR-triggered tumor progression remain to be fully understood.To evaluate the role of TLR in tumor cells.we investigated TLR4 signaling effects on human gastric cancer(GC).TLR4 expression in gastric cancer tissue (n=36)and cell lines(BGC，AGS，SGC，MGC，and MKN45)was detected by immunohistochemistry， RT-PCR,western blots，and FCM.Cell viability was analyzed with cell proliferation CCK8 kit according to the manufacture’S instruction.NF-kB localization data derived from immunohistological analysis graphed aus nucleus：cytoplasm (N∶C) ratio. The NF-kB luciferase reporter gene assay was used to detect the role of LPS on the activity of NF-kB.Supernatants of tumor cells were collected after 48 h of co-incubation with TLR ligands.LPS.
Xiangliang Yuan Weiwei Wang Lei Chen Yan Zhao Guohua Xie Tingting Rong Yanhui Ma Dakang Xu Lisong Shen
Department of Laboratory Medicine,Xinhua Hospital,Shanghai Jiaotong University School of Medicine,Sh Department of General Surgery,Xinhua Hospital,Shanghai Jiaotong University School of Medicine,Shangh Center for Cancer Research,Monash Institute for Medical Research,Monash University,Melbourne VIC 316
国内会议
中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议
北京
英文
243
2011-05-24（万方平台首次上网日期，不代表论文的发表时间）