Measurement of activities of enzyme labels in ELISA via an integration strategy to expand the quantifiable ranges：a prospect

摘要：

Enzyme-linked immunosorbent assay(ELISA) usually tolerates a nalTOW range to quantify an analyte of interest.In routine practice。activities of enzymes as labels in ELISA ale exclusively measured by the classical initial rate method using synthetic chromogenic substrates.Due to potential interference from contaminants in these chromogenic substrates，their cost and potential substrate.inhibition.practical substrate levels are always lower than those for saturating enzyme labels in ELISA.Therefore，ELISA inherently has a narrow quantifiable range of an analyte of interest.

作者: Xiaolan YANG Chun ZHANG Hongbo LIU Fei LIAO

作者单位: Key Laboratory of Medical Laboratory Diagnostics (the Ministry of Education) and the Department of Laboratory Medicine, Chongqing Medical University,Chongqing 400016,China

会议类型: 国内会议

会议名称: 中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

会议地点: 北京

会议语种:英文

页码: 260-261

在线出版日期: 2011-05-24（万方平台首次上网日期，不代表论文的发表时间）