Ezpression and Purification of Recombinant Bovine Neuropeptide Y in E.coli
Neuropeptide Y is a 36-amino acid peptide synthesized mainly in the hypothalamus and is one of the most potent orexigenic factors. To explore a approach for high expression of soluble NPY in Escherichia coli cells, we fused the sequence encoding bovine NPY gene to construct an expression vector pET32a(+)-bNPY. The fusion protein hexa-his-trx-bNPY was purified successfully by HisTrap HP affinity column. Immunohistochemical assays demonstrated that NPY was localized in cellular cytoplast and cellular membrane of neurons in cattle hypothalamic arcuate nucleus and the expressed bNPY protein obtained by splicing the gene in vitro can return to the cattle body itself. The procedure described in this study will provide a reliable and simple method for production of NPY to be used in animal production.
神经肽 融合表达 免疫组织化学 基因表达 养牛技术
Zhang Li Chen Hong Zhang Liang-zhi Zhang Ai-ling Zhang Qin
College of Animal Science and Technology,Northwest A&F University,Shaanxi Key Laboratory of Molecula College of Animal Science and Technology,Northwest A&F University,Shaanxi Key Laboratory of Molecula College of Animal Science and Technology,Northwest A&F University,Shaanxi Key Laboratory of Molecula
国内会议
青岛
英文
380-383
2008-10-18（万方平台首次上网日期，不代表论文的发表时间）