Single Nucleotide Polymorphisms of the ADRB3 Gene in Siz Chinese Sheep Populations detected by PCR-SSCP
The polymorphism of the Beta3-Adrenoceptors gene (ADRB3) has an association with lamb”s cold survival. To detect the Single Nucleotide Polymorphisms in the ADRB3 gene in Chinese sheep populations, we involved total 421 blood samples from 6 different Chinese local sheep populations (Hu, Small-tail Han, Ganjia, Ola, Qiaoke and Merino crossbred). The polymorphism of a 263bp region in the intron of the ADRB3 gene was analyzed by PCR-SSCP. As the result shows, total 12 novel SSCP patterns are detected. The sequences of these 5 new patterns identified by cloning and sequencing were proved as representatives of new ovine ADRB3 alleles (Genbank reference No. EU371403-EU371407). A deletion and 2 new Single Nucleotide Polymorphisms (SNPs) are deducted which add the number of SNPs in this region from 8 to 10. Furthermore, some of the new alleles were population-special which suggests they have potential link with population-special traits. In future, the association between polymorphism of the ADRB3 gene and cold tolerance therefore will been further confirmed by sheep”s cold-survival phenotype investigation in China high plateau region, so that the ADRB3 gene can be employed as a gene marker in lamb”s cold tolerance testing.
肾上腺素受体基因 耐寒性状 单核苷酸多态性 绵羊群体
Yuzhu Luo Guo Yang Huitong Zhou Jiang Hu Jon G H.Hickford
Faculty of Animal Science and Technology,Gansu Agricultural University,Lanzhou,China,730070 Gene-Marker Laboratory,Agriculture and Life Science Division,PO Box 84,Lincoln University,New Zealan
国内会议
青岛
英文
495-499
2008-10-18（万方平台首次上网日期，不代表论文的发表时间）