De novo Synthesis and Ezpression of a Thermostable Xylanase from Geobacillus stearothermophilus in Escherichia coli
The endoxylanase XT6 secreted by Geobacillus stearothermophilus is a particularly attractive candidate for some industrial purposes. The gene was de novo synthesized with the codons adjusted to fit the of that of Escherichia coli and constructed into vector pET28a(+). After optimizing the expression conditions，functional xylanase XT6 was over expressed in E.coli with up to 65％of total protein. A maximum xylanase activity of 3,030U/mL was obtained from cell extract against birchwood xylan. The recombinant XT6 was characterized. This is the first report on the over expression of a de novo synthesized xylanase XT6 gene Geobacillus stearothermophilus.
Geobacillus stearothermophilus Escherichia coli over ezpression zylanase
ZHANG Zhigang PEI Xiaoqiong WU Zhongliu
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China；Graduate School of Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
国内会议
成都
英文
1-13
2008-12-12（万方平台首次上网日期，不代表论文的发表时间）