Multiple dose pharmacokinetics of artemisinin in rats by high performance liquid chromatography-tandem mass spectrometry
Aim To investigate the pharmacokinetics of the antimalarial artemisinin in rats after single and multiple oral doses. Methods Six male Wistar rats were orally given a single dose of artemisinin 40 mg·kg-1(Group 1). Another six rats were orally given artemisinin at the same dose once daily for 5 consecutive days(Group 2). Artemisinin plasma concentrations，after single dose or after 5 consecutive doses，were determined by high performance liquid chromatography-tandem mass spectrometry(LC/MS/MS). Results The artemisinin pharmacokinetic parameters of Group 1 were compared with that of Group 2. The areas under the plasma concentration-time curve(AUC0-8)decreased from 126.53 h·ng·mL-1 of Group 1 to 39.91 h·ng·mL-1 of Group 2(p<0.05)，and oral clearance(CL/F)increased dramatically from 375.54 L·h-1 to 1365.84 L·h-1(p<0.05). However，the half-life(t1/2)did not change significantly(p>0.05). Conclusions The optimized LC/MS/MS method was successfully applied to the pharmacokinetic study of artemisinin in rats after single and multiple oral doses. The results suggested that artemisinin exhibits time-dependent pharmacokinetics.
时间依赖性 药代动力学 青蒿素 液相色谱-质谱分析法
Hong-Xia Yan Jie Xing Li-Feng Zhang Rui-Li Wang Shu-Qiu Zhang
School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, China;2Shanxi tumor hospital, Taiyua School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China School of Pharmacy, Shanxi Medical University, Taiyuan, 030001, China
国内会议
石家庄
英文
1-11
2008-10-18（万方平台首次上网日期，不代表论文的发表时间）