多糖修饰的巨噬细胞靶向超氧化物歧化酶及其体外降低辐射诱导的炎性细胞因子表达作用
To improve the ability of superoxide dismutase(SOD)to suppress reactive oxygenspecies(ROS)-mediated injury，chemically modified derivatives of SOD with N,N,Ntrimethyl chitosan chloride(TMC)and heparin，cationized SOD(TMC-SOD)and anionized SOD(heparin-SOD)，were designed and prepared. In this study，the inhibitory effect of TMC-SOD and heparin-SOD on superoxide anion release from macrophages was studied in vitro. Both TMC-SOD and heparin-SOD exhibited excellent inhibitory effects on superoxide anion release from macrophages，and the effects of TMC-SOD surpassed those of native SOD and heparin-SOD. The effects of TMC-SOD and heparin-SOD on inflammatory cytokine expression in vitro were also evaluated. The results showed that both TMC-SOD and heparin-SOD could significantly lower the levels of transforming growth factor-b1(TGF-b1)and interleukine-1b(IL-1?b)expressed by irradiated 3T3 fibroblasts. These results demonstrated that cationic polysaccharide or anionic polysaccharide SOD derivatives might be useful in the prevention and treatment of ROS-mediated inflammatory diseases. This study also demonstrated that chemical modification of SOD，especially cationization，greatly enhanced SOD￠s intracellular delivery，and as a consequence，produced a significant protective effect against ROSmediated injury.
超氧化物歧化酶 辐射诱导 化学改性 多糖修饰 炎性细胞因子
刘金峰 滕莉 刘纯慧 胡立宽 王永刚 刘宏 王凤山
山东大学药学院生化与生物技术药物研究所;山东大学国家糖工程技术研究中心 山东大学药学院生化与生物技术药物研究所 山东大学齐鲁医院肿瘤防治研究中心山东济南250012
国内会议
石家庄
中文
1-29
2008-10-18（万方平台首次上网日期，不代表论文的发表时间）