Studies on Antineoplastic Constituents from Marine Bryozoan Bugula neritina Inhabiting South China Sea: Isolation and Structural Elucidation of a Novel Macrolide Bryostatin 19
Objective: To investigate the antineoplastic constituents from marine bryozoan Bugula neritina inhabiting South China Sea. Methods: Antineoplastic compounds were screened by bioassay-guided method with a combination of extraction and partitionation as well as multiple column chromatographies (Sephadex LH-20, ODS and preparative HPLC). Structures of active compounds were established by employing high-resolution 2D NMR (600 MHz) and ESI-MS techniques. Results: A new antineoplastic macrolide bryostatin 19 (1) was isolated from this animal. Five known bryostatins: bryostatin 4 (2), bryostatin 5 (3), bryostatin 6 (4), bryostatin 18 (5) and bryostatin 10 (6), were for the first time obtained from this bryozoan in South China Sea. Conclusion: Bryostatin 19 (1) showed significant inhibition activity against U937 cell line with ED50 value of 3.2 nmol/L in vitro.
草苔虫 抗肿瘤活性 大环内酯
LIN Hou-Wen LIU Gao-Lin YI Yang-Hua YAO Xin-Sheng WU Hou-Ming
Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China Center of Marine Drugs,School of Pharmacy,Shanghai 200433 Shengyang Pharmaceutical University,Shengyang 110015 Shanghai Institute of Organic Chemistry,Chinese Academy of Science,Shanghai 200032
国内会议
全国首届海洋生物化学与分子生物学学术会议暨全国第五届海洋生命活性物质与天然生化药物学术研讨会
湖北宜昌
英文
276-283
2004-05-28（万方平台首次上网日期，不代表论文的发表时间）