Effect of starfish sterol screened by human arrhythmic related gene on ezperimental atrial fibrillation
To study the effectt of modified starfish steroid C03 screened by human arrhythmic related gene Kv1.5 on experimental atrial fibrillation. METHODS: Kv1.5 cDNA was translated into mRNA and microinjected into Xenopus oocyte. Models of atrial fibrillation was induced by electricity and drug. RESULTS: Pure and single IKur K+ current was expressed on oocytes, without other current pollution. Active material starfish steroid C03 blocking IKur current was screened by patch-clamp . C03 has demonstrated significantly and dose-dependently, to increase AFT induced by electricity in cats, AFT of C03 34.6mg ·kg-1 increased from (4.78 ±0.94)V to (10.87±2.38)V (P<0.01), which is better than Amio; shorten time of improving and recovering induced by Aco, C03 2.7, 8.1 and 24.2mg ·kg-1 shortened the improving time (P<0.01). C03 8.1 and 24.2 mg ·kg-1 shortened the recovering time (P<0.01); shorten time of improving and recovering induced by Ach, C03 8.1 and 24.2 mg ·kg-1 shortened the improving time (P<0.05),C03 2.7, 8.1 and 24.2 mg ·kg-1 shortened the recovering time (P<0.01). CONCLUSION:Starfish sterol C03 produced a oharmacological effects against experimental atrial fibrillation. The model of application of arrhythmic related gene could be used for screening of class III antiarrhythmic drugs from ocean active materials.
基因筛查 海星甾醇 抗心律失常 心房颤动
Xu Dong-Hui Zhu Ling Mei Xueting Xu Shi-Bo
School of Pharmaceutical Sciences,Sun Yat-sen University,Guangdong Guangzhuo 510275
国内会议
全国首届海洋生物化学与分子生物学学术会议暨全国第五届海洋生命活性物质与天然生化药物学术研讨会
湖北宜昌
英文
145-149
2004-05-28（万方平台首次上网日期，不代表论文的发表时间）