Norharman as the cytotozic principle in marine bacterium strain NJ6-3-1 associated with marine sponge Hymeniaci don perleve
Twenty nine marine strains were isolated from sponge Hymeniacidon perleve at Nanji island, and cytotoxic screening shown two strains show activity to Hela or BGC-823 cancer cell line. The active strains NJ6-3-1 and NJ6-3-2 were identified to be Pseudoalteromonas piscicida and Bacillus megaterium based on their 16s rRNA sequence analysis. The major cytotoxic metabolite of NJ6-3-1, isolated through bioassay-guide fractionation of cytotoxic assay, was identified as norharman (beta-carboline alkaloid) by EI-MS and NMR. TUNEL assay and flow cytometric analysis supposed that norharman exert cytotoxic action through apoptosis mechanism in Hela cells.
海洋细菌 海绵 细胞毒性
Xiaojun Yan Li Zheng Haimin Chen Pei Luqing
宁波大学海洋生物工程重点实验室,宁波 315211
国内会议
全国首届海洋生物化学与分子生物学学术会议暨全国第五届海洋生命活性物质与天然生化药物学术研讨会
湖北宜昌
英文
135-144
2004-05-28（万方平台首次上网日期，不代表论文的发表时间）