Ezpression, Purification, and Initial Characterization of GST-fusion v-SRC from a Bacterial Ezpression System
v-Src is a non-receptor protein tyrosine kinase, which is involved in many signal transduction pathways and closely related to the activation and/or development of cancers. Here we presentedthe expression, purification and initial characterization of GST-fusion v-Src in plsmid pLysS from abaterial expression system. Different culture conditions were examined in the indution of IPTG andGSH affinity chromatography was used to purified v-Src protein. With the versatile sub- strate poly-(Glu:Tyr) 4:1, ELISA was employed to ditermine the phosphorylation kinase activity of GST-fusedv-Src. This strategy seems to be more promise than the insect cell system or other eukaryotic systemsemployed in earlier Src expression.
酪氨酸蛋白激酶 信号转导 酶联免疫吸附试验 蛋白表达 癌症发生
GONG Xing-guo(龚兴国) ZHONG Wen-tao(钟文涛) Xie Jie(谢捷) Zhou Yuan(周远)
Institute of Biomacromolecule & Enzyme Engineering,College of Life Sciences,Zhejiang University,Hangzhou 310027,China
国内会议
杭州
英文
106-113
2004-10-01（万方平台首次上网日期，不代表论文的发表时间）