Effect of different selenium sources and levels on porcine circovirus type 2 replication in vitro
Porcine circovirus type 2(PCV2) has been linked to several disease syndromesduring the last decade. A deficiency in selenium has also been associated with the increases ofvirulence of some viruses and severity of infectious disease. In order to evaluate the effect ofdifferent selenium sources and levels on PCV2 replication in PK-15 cells,three selenium sources,i.e. sodium selenite,Kappa-selenocarrageenan and DL-selenomethionine at concentrations of 0,2,4,8,and 16 μmol/L were used throughout this experiment. PCV2 loads in PK-15 cells were measuredby a newly developed real-time quantitative PCR. A significantly inhibitive effect ofDL-selenomethionine on PCV2 replication in vitro was demonstrated and the inhibition wasconcentration-dependent within the range of 2-16 μmol/L. The inhibitive effect ofDL-selenomethionine on PCV2 replication may be caused by enhanced activity of glutathioneperoxidase. Our results may serve as a basis for further studies of the biological function ofselenium and control of PCV2 infection.
猪传染病 猪圆环病毒2型 无机硒源 有机硒源 硒水平 PCV2感染
Qunxing Pan Kehe Huang Kongwang He Fuzeng Lu
College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Institute of Vet College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
国内会议
广州
英文
740-748
2008-11-01（万方平台首次上网日期，不代表论文的发表时间）