Comparison of viability and infectivity of Cryptosporidium parvum oocysts stored in potassium dichromate solution and chlorinated tap water
The present study was undertaken to compare the viability and infectivity ofCryptosporidiumparvum oocysts that had been stored for 1,4,7,10,13,16,20,25 and 30 months at4℃ in 2.5％ potassium dichromate(Cr) and chlorinated tap water,respectively. An excystationprotocol was performed in vitro to evaluate viability. One hundred and eighty female BABL/c micewere used to evaluate the infectivity of oocysts by detecting the prepatent period of C. parvuminfection,the quantity of oocysts excreted,and the number of parasites that colonized the villi of theileum. The results showed that C. parvum oocysts preserved in Cr for 1-16 months and in water for1-13 months were capable of excystation in vitro and infection of mice in vivo. The excystation ratesof oocysts and the prepatent periods in mice infected by oocysts stored in Cr and water were notsignificantly different(p>0.05),and there was a strong correlation between prepatent period andduration ofoocyst storage(Cr: Re=0.92； water. R2=0.98). There were no significant differences inoocyst shedding from feces or parasitism of the terminal ilea of mice by Cryptosporidia between thetwo storage media(p>0.05). In conclusion,C. parvum oocysts may be stored at 4℃ in water insteadof Cr for the purposes of laboratory research. However,the presence of viable C. parvum oocysts inwater is a severe challenge to the drinking water treatment industry.
重铬酸钾 生活能力 绒毛寄生生物 体内传染 氯化自来水 卵囊储存
Fu Chen Kehe Huang Shunyi Qin Yuxin Zhao Cuiling Pan
College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China
国内会议
广州
英文
685-694
2008-11-01（万方平台首次上网日期，不代表论文的发表时间）