Isolation and Identification of the Kidney Tonic Constituents of Gu-Sui-Bu(Drynaria fortunei J.Sm.)
OBJECTIVE：To study the kidney tonic components of Rhizoma Drynariae.METHODS：The herbal material was extracted with 50％ ethanol.Silica gel and sephadex LH-20 coluuln chromatography isolated the chemical components using the Opossurn Kidney cell for the living model.The structures were identified on the basis of chemical and spectroscopic data.RESULTS.Six monocases were isolated from the EtOH extract of in Rhizomes of Drynaria fortunei J.Sm..Four structures were identified as naringin(I)，neocriocitrin(Ⅱ)，kaempferol(Ⅲ)，and naringenin-7-O-β-D-glucoside(Ⅳ)，respectively，on the basis of its spectral and physicochemical evidence.The kaempferol call promote the proliferation of the Opossum Kidney cell tested by Promega’B CellTiter96 assay in vitr0.Others are in identifying.CONCLUSION：Compound In and IV are firstly isolated from this plant，and the later may hefurther developed into a new effective drug for treatingm rural failure.
叶表面结构 骨碎补 柚皮苷 山奈酚 补肾成份
LI Shun-xiang LONG Mian YUE Chun-hua HUANG Dan ZHANG Zhi-guang
Hunan Provincial Key Laboratory of Drug Research,Hunan Academy of Traditional Chinese Medicine,Chang Hunan Agricultural University,Changsha 410128 State University of New York,Stonybrook NY11794 Hunan Provincial Key Laboratory of Drug Research,Hunan Academy of Traditional Chinese Medicine,Chang Hunan Agricultural University,Changsha 410128;Hunan Normal University,Changsha 410081
国内会议
南昌
英文
153-158
2007-12-01（万方平台首次上网日期，不代表论文的发表时间）