Galectin-1 Induces Immune Response and Antiviral Ability in Cherry Valley Ducks After Duck Plague Virus Infection
Valley ducks revealed that the coding sequence of duck galectin-1(duGal-1)comprises 405 bp,encoding 134 amino acids.Homologic analysis revealed its amino acid sequence is most identical to that of Anas platyrhynchos(98.8%)followed by Gallus gallus.Quantitative real-time PCR analysis indicated that duGal-1 mRNA is broadly expressed in healthy Cherry Valley duck tissues,primarily in the heart and trachea but minimally in the lung and skin.Meanwhile,the duGal-1 expression is slightly upregulated in the infected liver and spleen.Furthermore,the expression levels of ISGs(Mx,PKR,OAS)and some cytokines such as IFN-α,IL-1β,IL-2,are up-regulated to varying degrees after overexpression the duGal-1,In contrast,Knockdown of duGal-1 found that the expression levels of ISGs and some inflammatory cytokines were down-regulated.Antiviral assay showed that duGal-1 could inhibit viral replications early during infection.This is the first study of the cloning,tissue distribution,and antiviral immune responses of duGal-1,and findings imply it is involved in the early stages of antiviral innate immune responses to duck plague virus infections in ducks.
Galectin-1 Biological Function Immune Response Antiviral Cytokines
Shaojie Han Rong Li Jing Lin Xiaolan Hou Hongyu Weng Huihui Zhang Yanhua Wang Ning Li Tongjie Chai Liangmeng Wei
College of Animal Science and Veterinary Medicine,Sino-German Cooperative Research Centre for Zoonos College of Animal Science and Veterinary Medicine,Sino-German Cooperative Research Centre for Zoonos
国内会议
成都
英文
35-45
2019-05-29（万方平台首次上网日期，不代表论文的发表时间）