Fatty acids modulate the expression of pyruvate kinase and arachidonate-lipoxygenase through PPARγ/CYP2C45 pathway:a link to goose fatty liver
As cytochrome P-450 2C45(CYP2C45)is the most highly expressed cytochrome P-450 isoform in chicken liver,it may play an important role in avian liver biology.However,information regarding the function of CYP2C45 in goose fatty liver is generally limited.The aim of this study was to investigate the role of CYP2C45 in the development of goose fatty liver.Data indicated that CYP2C45 was induced in goose liver upon overfeeding for 19 days(P < 0.05).In goose primary hepatocytes,this gene was induced by insulin; the fatty acids oleate,linoleate,and palmitate; bezafibrate,a pan-peroxisome proliferator-activated receptor(PPAR)agonist; and troglitazone,a PPARγ agonist(P < 0.05).CYP2C45 overexpression and troglitazone induced the expression of pyruvate kinase(PK)and arachidonate 5-lipoxygenase(ALOX5),whereas troglitazone-induced upregulation was suppressed by small interfering RNAs targeting CYP2C45(P < 0.05).Additionally,PK and ALOX5 were upregulated in goose liver upon overfeeding(P < 0.05).These findings suggest that fatty acids induce CYP2C45 upregulation via PPARγ and that the induction of PK and ALOX5 in goose fatty liver is at least partially attributed to fatty acid-induced expression of CYP2C45,thus providing insight into the mechanism by which glycolysis and arachidonic acid metabolism are modulated in goose fatty liver.
CYP2C45 fatty acid PPAR pyruvate kinase arachidonate 5-lipoxygenase
M.M.Zhao T.J.Liu Q.Wang R.Zhang L.Liu D.Q.Gong T.Y Geng
College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China Poultry Institute,Chinese Academy of Agricultural Sciences,Yangzhou 225125,China;College of Animal S
国内会议
江苏扬州
英文
50-75
2019-04-01（万方平台首次上网日期，不代表论文的发表时间）