Genotyping and SNPs analysis of bovine leukemia virus in Chinese dairy cattle
Bovine leukemia virus(BLV)causes enzootic leucosis in cattle and is classified into 10 genotypes with a worldwide distribution,except for several European countries,Australia and New Zealand.Although BLV is widely spread in Chinese cows with the positive rate of 49.1%at individual level,very little is known about the BLV genotype in dairy cattle in China.To determine the BLV genetic variability in cows in China,112 BLV-positive samples from five cities in China were used for BLV molecular characterization in this study.Phylogenetic analysis using the Neighbor-Joining(NJ)method on partial env sequence encoding gp51 obtained from five Chinese cities and those available in GenBank(n=53,representing BLV genotype 1-10)revealed the Chinese strains belonged to genotype 6.Seven unique single nucleotide polymorphisms(SNPs)were identified among Yancheng,Shanghai and Bengbu strains out of the total 12 SNPs identified in Chinese strains.The genotyping coupled with SNPs analysis of BLV can serve as a useful molecular epidemiological tool for tracing the source of pathogen.This study highlights the importance of genetic analysis of geographically diverse BLV strains to understand BLV global genetic diversity.
bovine leukemia virus genotype single nucleotide polymorphisms Chinese dairy cattle
Yi Yang Shuangfeng Chu Shaobin Shang Zhangping Yang Chengming Wang
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,Jiangsu,China College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,Jiangsu,China;College of Animal S International Corporation Laboratory of Agriculture and Agricultural Products Safety,Yangzhou Univer
国内会议
江苏扬州
英文
76-85
2019-04-01（万方平台首次上网日期，不代表论文的发表时间）